Scheme：
Systemic and Temperature-sensitive Overexpression：
◉Technical difficulties：When GAL4 is driven by a strong systemic promoter, exogenous genes downstream of UAS will be overexpressed in the whole body of Drosophila, which may result in toxicity, leading to decreased survival ability or even death of the flies.
◉Solution：Fungene has provided Tubp-GAL4,Tubp-GAL80ts strain. Transgenic flies can survive and expand at low temperatures, while also ensuring overexpression of exogenous genes after high temperature heat-shock.
Technical Service Process:
other GAL4-UAS line construction：
In addition to the Tubp-GAL4,Tubp-GAL80ts, we can also provide multiple GAL4 strains, and are responsible for constructing stable lines with UAS flies, achieving spatiotemporal specific stable expression of exogenous genes in Drosophila.
Technical Service Process:
